Abstract:Tuwa people are one of the ancient nomads in northern Xinjiang. The cross-border life and the nature of small ethnic groups make it of great political and cultural significance to study and investigate them. Based on the related research of traditional settlement landscape gene map, three relatively well-preserved Tuwa grassland settlements in China were selected as the research objects. The landscape gene system of three settlements, namely“space”, “geography”and“culture”, was systematically constructed and identified at multiple scales, paired and interpreted. Based on this analysis, the mutation phenomenon in current settlements can be repaired and improved. The aim is to comb and improve the current theory and method of traditional residential landscape gene genealogy division, enrich the gene pool of traditional settlements in China, and provide reliable and reasonable reference basis for the restoration of genetic information chain of nomadic grassland settlements and the identification of landscape gene region, and provide scientific basis for the protection and development of herdsmen settlement.